The Compact Group of Galaxies HCG 31 is in an early phase of merging

We have obtained high spectral resolution (R = 45900) Fabry-Perot velocity maps of the Hickson Compact Group HCG 31 in order to revisit the important problem of the merger nature of the central object A+C and to derive the internal kinematics of the candidate tidal dwarf galaxies in this group. Our main findings are: (1) double kinematic components are present throughout the main body of A+C, which strongly suggests that this complex is an ongoing merger (2) regions $A2$ and E, to the east and south of complex A+C, present rotation patterns with velocity amplitudes of $\sim 25 km s^{-1}$ and they counterrotate with respect to A+C, (3) region F, which was previously thought to be the best example of a tidal dwarf galaxy in HCG 31, presents no rotation and negligible internal velocity dispersion, as is also the case for region $A1$. HCG 31 presents an undergoing merger in its center (A+C) and it is likely that it has suffered additional perturbations due to interactions with the nearby galaxies B, G and Q.Comment: 5 pages + figures - Accepted to ApJ Lette

Spiral inflow feeding the nuclear starburst in M83, observed in H-alpha emission with the GHAFAS Fabry-Perot interferometer

We present observations of the nearby barred starburst galaxy, M83 (NGC5236), with the new Fabry-Perot interferometer GHAFAS mounted on the 4.2 meter William Herschel Telescope on La Palma. The unprecedented high resolution observations, of 16 pc/FWHM, of the H-alpha-emitting gas cover the central two kpc of the galaxy. The velocity field displays the dominant disk rotation with signatures of gas inflow from kpc scales down to the nuclear regions. At the inner Inner Lindblad Resonance radius of the main bar and centerd at the dynamical center of the main galaxy disk, a nuclear $5.5 (\pm 0.9) \times 10^8 M_\odot$ rapidly rotating disk with scale length of $60 \pm 20$ pc has formed. The nuclear starburst is found in the vicinity as well as inside this nuclear disk, and our observations confirm that gas spirals in from the outer parts to feed the nuclear starburst, giving rise to several star formation events at different epochs, within the central 100 pc radius of M83.Comment: Accepted for publication in ApJ Letters. High-resolution version can be found at http://www.astro.su.se/~kambiz/DOC/paper-M83.pd

Impact of Th1 CD4 Follicular Helper T Cell Skewing on Antibody Responses to an HIV-1 Vaccine in Rhesus Macaques.

Generating durable humoral immunity through vaccination depends upon effective interactions of follicular helper T (Tfh) cells with germinal center (GC) B cells. Th1 polarization of Tfh cells is an important process shaping the success of Tfh-GC B cell interactions by influencing costimulatory and cytokine-dependent Tfh help to B cells. However, the question remains as to whether adjuvant-dependent modulation of Tfh cells enhances HIV-1 vaccine-induced antienvelope (anti-Env) antibody responses. We investigated whether an HIV-1 vaccine platform designed to increase the number of Th1-polarized Tfh cells enhances the magnitude and quality of anti-Env antibodies. Utilizing a novel interferon-induced protein 10 (IP-10)-adjuvanted HIV-1 DNA prime followed by a monophosphoryl lipid A and QS-21 (MPLA+QS-21)-adjuvanted Env protein boost (DIP-10 PALFQ) in macaques, we observed higher anti-Env serum IgG titers with greater cross-clade reactivity, specificity for V1V2, and effector functions than in macaques primed with DNA lacking IP-10 and boosted with MPLA-plus-alum-adjuvanted Env protein (DPALFA) The DIP-10 PALFQ vaccine regimen elicited higher anti-Env IgG1 and lower IgG4 antibody levels in serum, showing for the first time that adjuvants can dramatically impact the IgG subclass profile in macaques. The DIP-10 PALFQ regimen also increased vaginal and rectal IgA antibodies to a greater extent. Within lymph nodes, we observed augmented GC B cell responses and the promotion of Th1 gene expression profiles in GC Tfh cells. The frequency of GC Tfh cells correlated with both the magnitude and avidity of anti-Env serum IgG. Together, these data suggest that adjuvant-induced stimulation of Th1-Tfh cells is an effective strategy for enhancing the magnitude and quality of anti-Env antibody responses.IMPORTANCE The results of the RV144 trial demonstrated that vaccination could prevent HIV transmission in humans and that longevity of anti-Env antibodies may be key to this protection. Efforts to improve upon the prime-boost vaccine regimen used in RV144 have indicated that booster immunizations can increase serum anti-Env antibody titers but only transiently. Poor antibody durability hampers efforts to develop an effective HIV-1 vaccine. This study was designed to identify the specific elements involved in the immunological mechanism necessary to produce robust HIV-1-specific antibodies in rhesus macaques. By clearly defining immune-mediated pathways that improve the magnitude and functionality of the anti-HIV-1 antibody response, we will have the foundation necessary for the rational development of an HIV-1 vaccine

An improved method for statistical studies of the internal kinematics of HII regions: the case of M 83

We present the integrated Halpha emission line profile for 157 HII regions in the central 3.4' x 3.4' of the galaxy M 83 (NGC 5236). Using the Fabry-Perot interferometer GHaFaS, on the 4.2 m William Herschel on La Palma, we show the importance of a good characterization of the instrumental response function for the study of line profile shapes. The luminosity-velocity dispersion relation is also studied, and in the log(L)-log(sigma) plane we do not find a linear relation, but an upper envelope with equation log(L)=0.9 *log(sigma)+38.1. For the adopted distance of 4.5 Mpc, the upper envelope appears at the luminosity L=10^38.5 ergs, in full agreement with previous studies of other galaxies, reinforcing the idea of using HII regions as standard candles.Comment: 13 pages, 9 figures, accepted for publication in MNRA

Total dose effects on negative voltage regulator

Abstract Functional failure at low dose level (4 Krad(Si)) on voltage regulators (LM137) from different manufacturers are analysed. Dose rate effects on parts hardness are evaluated, showing that lowering the dose rate degrade more the ICs in the range 55 rad(Si)/s-0,s rad(Si)/s A failure mechanism is proposed, mainly based on circuit analysis, voltage contrast measurements, local irradiation and local electrical measurements with probe station. A spice simulation was performed, providing quantitative informations on the degradation. In the light of such a failure analysis and dose rate Pagure (Saclay, France) panoramic Cobalt60 source of 20 000 Ci located few kilometres away from VBlizy plant. Remote testing were performed in VBlizy using LTS 2020 automatic tester from Analog Devices, Digital Signal Analyser DSA601 from Tekmnic and HP4172 from Helwett Packard. Delay between irradiation and remote testing is kept below one hour. Local irradiation are performed using scanning transmission electron microscope JEOL JSM840 with electron energy up to 40 Kev. Contrast voltage potential measurement were carried out using a IDS Shlumberger tester. Probing measurements were performed using Wentworth probe station MP900 and HP4145 from Helwett Packard. effects, practical implications on radiation assurance are discussed. FAILURE DESCRIPTION I. INTRODUCTION Bipolar linear ICs are usually known as quite hard parts regarding Total Dose effects, with typical dose hardness in the range 50 to 100 Krad(Si). Such a postulate comes mainly from the relative intrinsic high hardness of bipolar transistor and passive devices. The problem is that bipolar transistors and passive elements used in linear ICs are not similar to the well known elemental structures. Because of integration constraint and in order to minimise the number of mask steps during IC processing, PNP transistors are often lateral ones. Such a type of transistor is already known as Total Ionizing Dose (TID) sensitiveIl1. When using such a sensitive structure in critical function of linear IC, loss of functionality can be foreseen, as already predicted by Johnston 2. In this paper, we will focus on negative voltage regulator (LM137 type) from 4 different manufacturers. We will analyse the degradation mode, based on different test methods. LM137 is a three terminal adjustable output negative voltage regulator. Reference voltage of -1.250V can be delivered for input voltage (referred to output voltage) ranging from -4.2% to -41.25V. The 137 circuit, shown in figure 1, consists in start up circuit, intemal comparator, band gap reference, power output stage and prowtion circuitry. ILEXPERIMENTAL PROCEDUR

Targeted delivery of bleomycin to the brain using photo-chemical internalization of Clostridium perfringens epsilon prototoxin

Cells infiltrating into normal brain from malignant brain tumors are protected by the blood brain barrier (BBB) which prevents the delivery and limits the effects of anti-tumor agents. We have evaluated the ability of photochemical internalization (PCI) to limit the effects of an agent known to broadly open the BBB to a target region of the brain. The PCI-based relocation and activation of macromolecules into the cell cytosol has the advantage of minimal side effects since the effect is localized to the area exposed to light, allowing the access of chemotherapeutic agents only to these regions. Non tumor bearing inbred Fisher rats were treated with photosesitizer, and a nontoxic intraperitoneal dose of Clostridium perfringens epsilon prototoxin (ETXp) followed by light exposure. Post-contrast T1 MRI scans were used to monitor the degree BBB disruption. F98 tumor cells were implanted into the brains of other animals that were subsequently treated 24 h later with ETXp-PCI BBB opening followed by the i.p. administration of bleomycin (BLM). PCI delivery of ETXp at low fluence levels demonstrated significant MRI enhancement. No effect on the BBB was observed if photosesitizer and light was given in the absence ETXp. The survival of animals implanted with F98 tumor cells was significantly extended following ETXp-PCI BBB opening and BLM therapy compared to controls. PCI delivered ETXp was effective in opening the BBB in a limited region of the brain. ETXp-PCI mediated BBB opening clearly increased the efficacy of BLM therapy

The Brazilian Tunable Filter Imager for the SOAR telescope

This paper presents a new Tunable Filter Instrument for the SOAR telescope. The Brazilian Tunable Filter Imager (BTFI) is a versatile, new technology, tunable optical imager to be used in seeing-limited mode and at higher spatial fidelity using the SAM Ground-Layer Adaptive Optics facility at the SOAR telescope. The instrument opens important new science capabilities for the SOAR community, from studies of the centers of nearby galaxies and the insterstellar medium to statistical cosmological investigations. The BTFI takes advantage of three new technologies. The imaging Bragg Tunable Filter concept utilizes Volume Phase Holographic Gratings in a double-pass configuration, as a tunable filter, while a new Fabry-Perot (FP) concept involves technologies which allow a single FP etalon to act over a large range of interference orders and spectral resolutions. Both technologies will be in the same instrument. Spectral resolutions spanning the range between 25 and 30,000 can be achieved through the use of iBTF at low resolution and scanning FPs beyond R ~2,000. The third new technologies in BTFI is the use of EMCCDs for rapid and cyclically wavelength scanning thus mitigating the damaging effect of atmospheric variability through data acquisition. An additional important feature of the instrument is that it has two optical channels which allow for the simultaneous recording of the narrow-band, filtered image with the remaining (complementary) broad-band light. This avoids the uncertainties inherent in tunable filter imaging using a single detector. The system was designed to supply tunable filter imaging with a field-of-view of 3 arcmin on a side, sampled at 0.12" for direct Nasmyth seeing-limited area spectroscopy and for SAM's visitor instrument port for GLAO-fed area spectroscopy. The instrument has seen first light, as a SOAR visitor instrument. It is now in comissioning phase.Comment: accepted in PAS